Table 3.

Examples of Leishmania targets used in HTS campaigns.

Protein	Abbreviation	Target limitations	Reference
Casein kinase 1.2	LmCK1.2	High degree of conservation between the parasite and human CK1 isoforms represents a challenge for the identification of parasite-specific CK1 inhibitors with limited side effects on host kinases (Rachidi et al., 2014).	(Durieu et al., 2016)
N-myristoyltransferase	NMT	Very few proteins have been experimentally validated as N-myristoylated in Leishmania species, impairing the understanding of its essentiality in parasite biology (Tate et al., 2014)	(Bell et al., 2012)
Cdc2-related kinase 3	CRK3	Poor correlation between potency against the target and anti-parasitic activity, suggesting some unknown aspect of CRK3 biology in Leishmania or unknown bioavailability within the parasitophorous vacuole (Jones et al., 2018)	(Walker et al., 2011)
Pteridine reductase	PTR1	Despite extensive work, no inhibitors for this target have been progressed to preclinical development (Field et al., 2017)	(Cavazzuti et al., 2008)