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. 2018 Sep 7;9(10):1698–1707. doi: 10.1039/c8md00257f

Fig. 2. Hyperpolarization of the bacterial cytoplasmic membrane. A) The fluorescence of the dye DiSC3(5) within a MRSA (JCSC 4788) bacterial suspension was monitored with a spectrophotometer. B) The use of SYTOX Green demonstrated that treatment with over 1× MIC RDSB 11k for 3 h induced membrane disruption of MRSA (JCSC 4788). In contrast, no increase in fluorescence was observed when vancomycin was added. Melittin was used as a positive control. Error bars represent the standard deviation, n = 3. C) Compound 11k compromised the inner membrane of MRSA and caused significant increases in fluorescence emission due to SYTOX Green binding to nucleic acids. D) In contrast, no significant fluorescence emission was observed, when MRSA was treated with vancomycin.

Fig. 2