Skip to main content
. Author manuscript; available in PMC: 2018 Oct 19.
Published in final edited form as: Nat Protoc. 2017 Jan 26;12(2):439–451. doi: 10.1038/nprot.2016.174

Figure 4 |.

Figure 4 |

Modification of standard CR conditions for certain tissue-derived cells. CR cell cultures from pancreas, colon and neuroendocrine cervical carcinoma exhibited unique morphologies and required modifications of standard CR culture conditions for optimal proliferation (Table 1). The CR neuroendocrine cervical carcinoma cells form tumors in nude mice equivalent to the primary tumor (data not shown). The differentiation of pancreas and colon CR cells has not been evaluated. Scale bars, 200 μm (colon), 400 μm (pancreas and neuroendocrine cervical carcinoma). Tissue specimens were collected with the informed consent of patients according to Georgetown University Institutional Review Board protocols.