Figure 5 |.

Evaluation of paired tumor and normal biopsies. Thirty-seven cases of primary prostate cancer were enrolled in the present study. A board-certified pathologist at Georgetown University Medical Center (B.K.) collected biopsies from four sites for each case: two that were deemed malignant and two that were deemed normal. For each biopsy, slices were made for CR culture and for histological evaluation. Using hematoxylin to stain proteins, filaments and intracellular membranes (pink) and eosin to stain nuclei and nucleic acids (blue), the percentages of tumor cells in the biopsies were determined according to standard histopathological criteria (Table 2). Representative histological images are shown for patient PP-004. Scale bars, 100 μm; scale bars apply to images directly above. Tissue specimens were collected with the informed consent of patients, according to Georgetown University Institutional Review Board protocols.