TABLE 1 |.

CR cell growth conditions.

	Cell culture conditions
	Standard CR coculture			Conditioned Medium	2% O2	Collagen coating	Poly L-ornithine coating	B-27	R-spondin	N-2 Supplement
Tissue type	F medium	J2 cells	Y-27632
Breast (N, T)	+	+	+
Prostate (N, T)	+	+	+
Lung (N, T)	+	+	+
Cervix (N, T)	+	+	+
Oral (N, T)	+	+	+
Ovary (N, T)	+	+	+
Skin (N, T)	+	+	+
Salivary gland (N,T)	+	+	+
Kidney (N, T)	+	+	+
Colon (T)	+		+		+	+			+
Colon (N)			+	+	+	+			+
Pancreas (N, T)	+		+		+	+			+
Thyroid (N, T)	+	+	+			+
Schwannomas			+	+			+	+		+
Neuroendocrine cells (T)	+		+			+
GIST cells (T)	+		+		+

Normal (N) and tumor (T) cells from various tissues, including pancreas, colon and neuroendocrine carcinoma (Fig. 4) require modifications of standard CR culture conditions for optimal proliferation. The concentrations of all components are listed in the ‘REAGENTS’ and ‘REAGENT SETUP’ sections, except for that for R-spondin, which is 1 μg/ml.