TABLE 1 |.
Cell culture conditions | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Standard CR coculture | Conditioned Medium |
2% O2 |
Collagen coating |
Poly L-ornithine coating |
B-27 | R-spondin | N-2 Supplement |
|||
Tissue type | F medium | J2 cells | Y-27632 | |||||||
Breast (N, T) | + | + | + | |||||||
Prostate (N, T) | + | + | + | |||||||
Lung (N, T) | + | + | + | |||||||
Cervix (N, T) | + | + | + | |||||||
Oral (N, T) | + | + | + | |||||||
Ovary (N, T) | + | + | + | |||||||
Skin (N, T) | + | + | + | |||||||
Salivary gland (N,T) | + | + | + | |||||||
Kidney (N, T) | + | + | + | |||||||
Colon (T) | + | + | + | + | + | |||||
Colon (N) | + | + | + | + | + | |||||
Pancreas (N, T) | + | + | + | + | + | |||||
Thyroid (N, T) | + | + | + | + | ||||||
Schwannomas | + | + | + | + | + | |||||
Neuroendocrine cells (T) | + | + | + | |||||||
GIST cells (T) | + | + | + |
Normal (N) and tumor (T) cells from various tissues, including pancreas, colon and neuroendocrine carcinoma (Fig. 4) require modifications of standard CR culture conditions for optimal proliferation. The concentrations of all components are listed in the ‘REAGENTS’ and ‘REAGENT SETUP’ sections, except for that for R-spondin, which is 1 μg/ml.