Skip to main content
. 2018 Oct 19;13(10):e0205585. doi: 10.1371/journal.pone.0205585

Fig 5. Multi-cassette induced knockdown of all 4 proteins of the MEK1/2-ERK1/2 module.

Fig 5

(A) pT2-CAG-puro-tTR + pT2-neo-S2-shMEK2-shMEK1-shERK2-shERK1 (= pT2-neo-S2-MMEE) AMO-1 cells treated with either 1 or 2 μg/ml doxycyclin and harvested for Western blotting 3 and 5 days post-induction (D3/D5). D0 denotes the same cells harvested directly prior to doxycyclin treatment. (B) Similar experiment as in A but conducted with JJN-3 cells. Western blotting shown for cells harvested at day 5 post-induction with 1 or 2 μg/ml doxycyclin. All targets were stained on different blots, but with equal amounts of sample loaded from a single preparation. The respective tubulin loading controls are thus representative for all associated blots.