Table 4. Proteins of the phage Str01 detected by mass spectrometry.
no | NCBI Identical Protein Group | phage protein accession | phage protein defintion | score | % coverage | emPAI | |
---|---|---|---|---|---|---|---|
A | 1 | 23526059 | APZ81895 | major capsid protein | 7931 | 78.1 | 6.35 |
2 | 92043013 | APZ81902 | tail protein | 410 | 34.2 | 3.37 | |
3 | 5668030 | APZ81903 | hypothetical protein | 184 | 55.3 | 3.1 | |
4 | 23526069 | APZ81888 | portal protein | 769 | 39.0 | 1.7 | |
5 | 92043017 | APZ81889 | tail protein | 519 | 34.0 | 1.45 | |
B | 6 | 137081518 | APZ81883 | minor capsid protein | 113 | 35.2 | 0.98 |
7 | 23526073 | APZ81882 | holin | 150 | 43.2 | 0.94 | |
8 | 14467 | APZ81898 | nucleotide-binding protein | 135 | 22.1 | 0.91 | |
9 | 23526076 | APZ81901 | terminase small subunit | 139 | 18.2 | 0.58 | |
10 | 43836092 | APZ81891 | N-acetylmuramoyl-L-alanine amidase | 134 | 10.8 | 0.39 | |
11 | 23526079 | APZ81875 | minor capsid protein | 112 | 6.8 | 0.21 | |
12 | 137081495 | APZ81886 | tail tape measure protein | 183 | 4.9 | 0.15 |
Proteins are divided into ‘A’—“high abundance” and ‘B’—“low abundance” groups based on emPAI (Exponentially Modified Protein Abundance Index) parameter. Hit proteins were filtered based on: ions significance threshold (< 1e-5), ions expect cut-off (< 1e-5), overall protein score (> = 100) and emPAI (> = 0.15).