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. 2018 Oct 19;13(10):e0206169. doi: 10.1371/journal.pone.0206169

Fig 1. Schematic illustration of sequence of the designated vectors.

Fig 1

pET28b (+) vectors harboring fusion genes are depicted here. The positions of NcoI, NdeI and NotI restriction sites are also indicated. (A) and (B) respectively stand for the vectors including the fusion gene of αB-BC and αB-AC. The translation frame under the vectors correspond to the amino acid sequence of the fusion proteins. The sequence of αB-Cry fusion partner is indicated in blue. (M) stands for the single letter abbreviation of methionine serving as the CNBr cleavage site. The amino acid sequence of B- and A-chain of human insulin is indicated in red. Isoleucine and valine residues (in pink) generated by site direct mutagenesis in αB-Cry gene instead of methionine and proline, respectively.