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. 2018 Oct 19;13(10):e0206169. doi: 10.1371/journal.pone.0206169

Fig 2. The SDS–PAGE analysis of the fusion proteins expression and purification.

Fig 2

The protein samples in different steps of the purification of fusion proteins (αB-AC and αB-BC) were subjected to gel electrophoresis analysis. Lane 1 indicates molecular mass marker. Lanes 2, 3 and 4, respectively, stand for the supernatant, the pellet subjected to the subsequent washing with Triton X-100 and urea, and αB-AC fusion protein solubilized in 8 M urea. Lanes 5, 6 and 7 indicate SDS-PAGE analyses of protein in the similar steps during purification of αB-BC fusion protein.