Fig 4. SDS–PAGE analyses of the fusion proteins cleavage.
The fusion proteins were subjected to CNBr cleavage and the protein samples were analyzed by reducing SDS-PAGE (gel 18%). Lane 1 stands for the molecular mass marker. Lanes 2, 3 and 4 indicate αB-Cry, αB-AC and αB-BC, respectively. Lanes 5 and 6 show αB-BC and αB-AC fusion proteins after cleavage by the CNBr.