Fig 5. Gel filtration and SDS-PAGE analyses of the digested fusion proteins.

The digest products of two fusion proteins with CNBr were individually applied to a Sephadex G50 gel filtration column (1.6 x 94 cm). The experiments were done by dissolving αB-BC in 1 M acetic acid and αB-AC in 20 mM Tris containing 8 M urea at pH 8.0. The elution profile and reducing SDS-PAGE profile (gel 18%) (the insets) corresponding to αB-AC (A) and αB-BC (B) are indicated.