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RP-HPLC and SDS-PAGE analyses of A- and B-chain of human insulin. The purified samples of A- and B-chain of human insulin were subjected to RP-HPLC and SDS-PAGE analyses. RP-HPLC analysis was conducted on C18 analytical column for the A- and B-chain of both standard insulin (STD) and human recombinant insulin product (R). The inset also shows the SDS–PAGE profile (gel 18%) of the purified chains of human insulin.
