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. 2018 Oct 19;13(10):e0206169. doi: 10.1371/journal.pone.0206169

Fig 7. The chain combination analysis with RP-HPLC in the presence of αB-Cry.

Fig 7

αB-Cry chaperone was used to increase efficacy of the chain combination step. (A) Peaks 1–4, respectively, indicate the position of oxidized A-chain, native insulin, oxidized B-chain and intermediate products presumably with the incorrect disulfide linkage. (B) Peaks 1–5 stand for the oxidized A-chain, native insulin, oxidized B-chain, αB-Cry and presumably the intermediate insulin species with the incorrect disulfide linkage. In both (A) and (B), the peaks correspond to the native insulin are distinguished by the white solid diamond as indicated by authentic insulin in the (N) line. The experiments were repeated three times.