Table 3. Factors affecting the live birth rate after frozen-thawed blastocyst transfer: Logistic regression analysis of the risk.
| Univariate logistic regression analysis | Multiple logistic regression analysis* | |||||
|---|---|---|---|---|---|---|
| Parameters | Odds ratio | 95% CI | p-value | Odds ratio | 95% CI | p-value |
| Age > 35 y.o. at retrieval | 0.47 | 0.30–0.72 | 0.001 | 0.48 | 0.31–0.75 | 0.001 |
| Smoker | 1.22 | 0.63–2.36 | 0.560 | |||
| BMI > 30 | 0.40 | 0.34–1.52 | 0.393 | |||
| Secondary vs. primary infertility | 0.76 | 0.49–1.17 | 0.211 | |||
| IVF/ICSI rank£ | 0.353 | 0.759 | ||||
| One previous IVF/ICSI cycle vs. no previous IVF/ICSI cycle | 0.94 | 0.56–1.60 | 0.831 | 1.02 | 0.59–1.75 | 0.942 |
| ≥ 2 previous IVF/ICSI cycles vs. no previous IVF/ICSI cycle | 0.69 | 0.42–1.14 | 0.151 | 0.83 | 0.49–1.40 | 0.486 |
| Patient’s ovarian reserve: | ||||||
| Day-3 FSH > 8 (IU/L) | 0.82 | 0.44–1.53 | 0.528 | |||
| AMH < 2 ng/mL | 0.84 | 0.46–1.53 | 0.574 | |||
| AFC < 10 | 1.20 | 0.67–2.15 | 0.536 | |||
|
Peak estradiol levels (pg/mL) at triggering |
0.362 | 0.546 | ||||
| < 1,500a | 1.28 | 0.73–2.26 | 0.394 | 1.22 | 0.68–2.11 | 0.500 |
| > 2,500a | 1.42 | 0.88–2.28 | 0.154 | 1.31 | 0.81–2.14 | 0.272 |
| Type of triggering: | ||||||
| hCG vs. GnRH agonist | 1.48 | 0.86–2.57 | 0.158 | 1.38 | 0.79–2.41 | 0.258 |
| Nb. of embryos transferred (2 vs. 1) | 0.89 | 0.17–4.64 | 0.890 | |||
| Good quality embryo transfer b | 0.52 | 0.25–1.08 | 0.080 | 1.52 | 0.95–2.41 | 0.078 |
| Cycle of transfer (‘cycle ≥ 2’ vs. ‘1’) | 0.93 | 0.62–1.39 | 0.726 | 0.94 | 0.62–1.42 | 0.770 |
| Estradiol treatment | ||||||
| Oral vs. transdermal | 0.82 | 0.42–1.61 | 0.571 | |||
| Endometrial thickness c | 1.03 | 0.93–1.14 | 0.600 | |||
| Progesterone c | 0.68 | 0.30–1.53 | 0.350 | |||
y.o., years old; AMH, anti-Müllerian hormone; AFC, antral follicle count; FSH, follicle stimulating hormone; IVF/ICSI, in vitro fertilization / intracytoplasmic sperm injection; Nb, number
* Age (> 35 y.o.), IVF/ICSI rank, Peak estradiol levels (pg/mL) at triggering, Type of triggering, Embryo quality, and FBT cycle (cycle ≥ 2 vs. cycle 1) were included in the multiple logistic regression model.
£ - The number of previous IVF/ICSI cycles is defined as the number of previous controlled ovarian stimulations leading to at least one embryo transfer with no pregnancy being obtained.
a—Peak estradiol levels (pg/mL) at triggering comprised between 1,500 and 2,500 IU were considered as the reference.
b—A good-quality embryo was defined as a B3-B4 or B5 embryo ≥ BB according to the grading scale proposed by Gardner
c- on the 1st day of the progesterone administration