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. Author manuscript; available in PMC: 2019 Apr 20.
Published in final edited form as: ACS Chem Biol. 2018 Feb 20;13(4):933–941. doi: 10.1021/acschembio.7b01083

Figure 1.

Figure 1.

Effects of Hsp90 paralogs on mutant myocilin aggregation. a) Schematic of possible interactions between Hsp90 paralogs and aggregating mutant myocilin in a cellular context. b) Effects of Hsp90α, Hsp90β, and Grp94 siRNA knockdown on mutant myocilin levels, as well as ER stress markers Grp78 and pIRE1α. c) All Hsp90s enhance the rate of OLF aggregation in vitro. d) Post-assay analysis of aggregates from (c) by SDS-PAGE (right) reveals Hsp90α and Hsp90β co-aggregation with OLF. S = supernatant; W = wash; P = aggregate pellet. Left: Hsp90α or Hsp90β at the start of the aggregation experiment.