Table 1.
Hit Ranking and Properties of Blagg Lab Blind Screen Compounds
| Compound # | Tier of Inhibitor |
Chemical Structure | Isoform Selectivity | Domain Targeted | % Inhibitiona |
|---|---|---|---|---|---|
| 5 | Top |  |
Grp94 | N-term | 159.6 |
| 1 | Top |  |
Grp94 | N-term | 148.4 |
| 3 | Mid |  |
Grp94 | N-term | 54.3 |
| 7 | Mid |  |
Grp94 | N-term | 52.8 |
| 2 | Mid |  |
Grp94 | N-term | 52.3 |
| 11 | Mid |  |
Hsp90β | N-term | 43.2 |
| 10 | Low |  |
Grp94 | N-term | 31.4 |
| 4 | Low |  |
Non-selective | N-term | 28.4 |
| 6 | Low | Structure Not Disclosed | Hsp90α | N-term | 27.9 |
| 9 | Low | Structure Not Disclosed | Hsp90β | N-term | 27.4 |
| 13 | Non |  |
-- | C-term | −31.7 |
| 16 | Non |  |
-- | C-term | −32.0 |
| 12 | Non |  |
-- | C-term | −38.7 |
| 17 | Non |  |
-- | C-term | −43.5 |
| 14 | Non |  |
-- | C-term | −85.1 |
| 15 | Non |  |
-- | C-term | −86.7 |
| 8 | Non | Structure Not Disclosed | Hsp90α/β | N-term | −98.3 |
a
See Supporting Information for calculation of % Inhibition after correction for the heat gradient experienced and intrinsic compound fluorescence. Tier ranking: >66% inhibition (top), >33% (mid), >0% (low), <0% (Non) non-hit).