Table 1. Summary of all mice.
| Experimental group | Injection site | Behavioral assays | |||||
|---|---|---|---|---|---|---|---|
| Grooming | Social chamber | Y-maze | Elevated Plus Maze | Gait | Eyeblink | ||
|
Adult |
Lobule VI | 12 | 12 | 12 | 12 | 12 | 8 |
| Lobule VII | 10 | 10 | 7* | 10 | 10 | - | |
| Crus I | 10 | 10 | 10 | 10 | 10 | 8 | |
| Crus II | 11 | 11 | 11 | 11 | 11 | 6 | |
| Eyeblink | - | - | - | - | - | 9 | |
| *not counting three mice excluded from group comparison during habituation phase | |||||||
|
Developmental |
Lobule VI | 13 | 13 | 13 | 13 | 13 | 6** |
| Lobule VII | 8 | 8 | 8 | 8 | 8 | - | |
| Crus I | 7 | 7 | 7 | 7 | 7 | 6** | |
| Crus II | 12 | 12 | 12 | 12 | 12 | 3 | |
| **not counting four mice (1 crus I, 3 lobule VI) excluded due to eyeblink-zone spillover expression | |||||||
| Controls | Type | ||||||
|
Adult |
DREADDs (eyeblink zone)+saline injections | - | - | - | - | - | 5 |
| Saline i.p. | 9 | 9 | 9 | 9 | 9 | - | |
| CNO alone | 10 | 10 | 10 | 10 | 10 | 9 | |
| No treatment | 8 | 8 | 17 | 18 | 18 | 16 | |
| GCaMP6f injections + CNO | - | - | 8 | - | - | - | |
| Developmental | CNO alone | 10 | 10 | 10 | 10 | 10 | - |