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. 2018 Oct 2;9(77):34567–34581. doi: 10.18632/oncotarget.26179

Figure 2. Selinexor reduces bladder tumor cell the viability.

Figure 2

(A) Cell viability assays of four bladder tumor cell lines treated with different concentrations of selinexor for 72 hours. Error bars denote standard deviations. (B) Colony formation assays of four cell lines treated with 0.1 uM selinexor or vehicle for T24-12 days, UM-UC-3-11 days, J82-9 days and TCCSUP-10 days. At the end of the study, the cells were fixed with methanol and stained with crystal violet. (C) Expression of XPO1 in bladder tumor cell lines treated with 0.1 uM selinexor for 72 hours analyzed by Western immunoblots. Tubulin serves as a protein loading control. V = vehicle, S = selinexor. (D) Immunofluorescent detection of XPO1 (green) in cells treated with vehicle and selinexor. Cells were plated on chamber slides and they were treated with either vehicle or selinexor for 48 hours. Tubulin (red) and DAPI (blue) served to define the nuclear and cytoplasmic compartment, respectively. (E) Quantitation of XPO1 expression normalized to DAPI. *denotes p ≤ 0.05, **denotes p ≤ 0.01. S.E.M.