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. Author manuscript; available in PMC: 2018 Oct 19.
Published in final edited form as: Science. 2018 Apr 6;360(6384):eaar5078. doi: 10.1126/science.aar5078

Fig. 2. Calcium as well as TSG101 and ALIX are required for ESCRT assembly on damaged endolysosomes.

Fig. 2.

(A and B) HeLa cells transfected with the indicated siRNAs were treated with or without LLOME and stained for CHMP4A (A) or GAL3 (B). The proportion of each stain that exceeded a starting threshold is quantified (in the order shown: n = 20, 33, 21, and 33 cells in A; and 20, 21, 20, and 27 cells in B; n.s. denotes p = 0.6319 by Student’s two-tailed unpaired t-test). Horizontal bars mark the median; whiskers span the interquartile range. (C) U2OS cells producing mCherry-GAL3 were preincubated for 1 h with or without BAPTA-AM, then treated with DMSO or LLOME and stained for CHMP4A. Scale bars equal 10 µm.