Figure 5.

Inhibition of TG-sensitive SERCA1a Ca²⁺ ATPase activity by selected HOCs of marine origin. (A) Representative traces showing the oxidation of NADH in the coupled enzyme assay was monitored by measuring absorbance at 340−400 nm. Veh (0.1% DMSO), TG (thapsigargin, 20 μM) or a HOC was added into separate reactions cuvettes before addition of NADH and Na₂ATP to initiate the reactions. The oxidation rate of NADH in the presence of TG, Veh or test compounds were summarized in bar graph (B−G). Two different microsomal (JSR) preparations were used to calculate the summary data for each group, and each performed in triplicates or quintuplicates. Data shown as Mean ± SD and statistical differences assessed by one-way ANOVA, followed by Dunnett’s multiple comparisons test was performed using Graph Pad 7.03. * p < 0.05, **p < 0.01 vs Veh, ## p < 0.01 vs TG, and n.s. indicates no significant compared with Veh.