Table 1.

Primers used for genotyping by PCR.

Allele	Strand	Sequence	Amplicon Size (bp)
Lyz2Cre	Forward1 Forward2 Reverse	5′-TTACAGTCGGCCAGGCTGAC-3′	Lyz2WT = 350 Lyz2Cre = 700
		5′-CCCAGAAATGCCAGATTACG-3′ 5′-CTTGGGCTGCCAGAATTTCTC-3′
Nfatc2loxP	Forward Reverse	5′-GGTGTCTCCCTCAAATGTTCA-3′ 5′-GCGAATATGAGGACCCAAGT-3′	WT = 160 Nfatc2loxP= 250
Frt and LoxP recombination	Strand	Sequence	Amplicon Size (bp)
Frt recombination of Nfatc2 targeted allele	Forward Reverse	5′-AGTATGTAACCTCTGCTTCC-3′ 5′-AGCACAATGCCCATGTTCAC-3′	WT = 250 Not recombined = no band Recombined = 351 (Nfatc2loxP)
LoxP recombination of Nfatc2loxP	Reverse Forward	5′-GGTGTCTCCCTCAAATGTTCA-3′ 5′-AGCACAATGCCCATGTTCAC-3′	Not recombined = 2342 (Nfatc2loxP) Recombined = 358 (Nfatc2Δ)