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. Author manuscript; available in PMC: 2019 Oct 18.
Published in final edited form as: Mol Cell. 2018 Oct 4;72(2):328–340.e8. doi: 10.1016/j.molcel.2018.08.038

Figure 3. PA inhibits LATS1/2 kinases in the Hippo pathway.

Figure 3.

(A) PA inhibits LATS phosphorylation but not MST phosphorylation. Serum-starved HEK293A cells were treated by PA (100 μM) or serum for 30 min.

(B-D) PA inhibits YAP phosphorylation in the MST1/2 DKO, MOB1A/B DKO and NF2 KO cells. Wild-type HEK293A cells as well as the MST1/2 DKO (B), MOB1A/B DKO (C) and NF2 KO (D) cells, were serum starved for 24 hours and treated with PA (100 μM) for 30 min.

(E) Inhibition of PLD-mediated PA production fails to rescue YAP phosphorylation in the LATS1/2 DKO cells. Wild-type HEK293A and LATS1/2 DKO cells were treated by the indicated inhibitors (0.3% 1-Butanol, 0.3% 2-Butanol, 30 μΜ FIPI, and 20 μΜ CAY10594) for 1 hour.

See also Figure S2.