KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-NF-κB p65 | Cell Signaling Technology |
Cat#8242; RRID: AB_10859369 |
| peroxidase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
Jackson Immuno Research |
Cat#111–035-144; RRID: AB_2307391 |
| Lamin A/C Antibody | Cell Signaling Technology |
Cat#2032; RRID: AB_2307391 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| For detailed usage of chemicals, please see supplementary information. |
||
| SEAP reporter assay | Invivogen | Cat#rep-qb1 |
| WST-1 cell proliferation assay | Roche | Cat#5015944001 |
| Deposited Data | ||
| TLR8/CU-CPT9a structure | This paper | PDB: 5Z14 |
| TLR8/CU-CPT9b structure | Zhang, et al., 2018 | PDB: 5WYZ |
| TLR8/CU-CPT9c structure | This paper | PDB: 5Z15 |
| TLR8/CU-CPT8m structure | Zhang, et al., 2018 | PDB: 5WYX |
| Experimental Models: Cell Lines | ||
| Human: HEK-Blue-hTLR2 cells | Invovogen | Cat#hkb-htlr2 |
| Human: HEK-Blue-hTLR3 cells | This paper | N/A |
| Human: HEK-Blue-hTLR4 cells | Invovogen | Cat#hkb-htlr4 |
| Human: HEK-Blue-hTLR5 cells | This paper | N/A |
| Human: HEK-Blue-hTLR7 cells | Invovogen | Cat#hkb-htlr7 |
| Human: HEK-Blue-hTLR8 cells | This paper | N/A |
| Human: HEK-Blue-hTLR9 cells | Invovogen | Cat#hkb-htlr9 |
| Human: THP-1 | ATCC | Cat#TIB-202 |
| Experimental Models: Organisms | ||
| Splenocytes from hTLR8tg/TLR7-KO mice | This paper | N/A |
| Software and Algorithms | ||
| GraphPad Prism | GraphPad Software Inc |
https://www.graphpad.com/ scientific-software/prism/ |
| OriginPro | OriginLab Corporation | https://www.originlab.com/index.aspx?go=Products/Origin |