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. Author manuscript; available in PMC: 2019 Oct 18.
Published in final edited form as: Cell. 2018 Sep 20;175(3):665–678.e23. doi: 10.1016/j.cell.2018.08.049

Figure 2. Organ-specific maps of gut vagal afferents.

Figure 2

A-D. The retrograde Cre-carrying construct AAVrg-pmSyn1-EBFP-Cre was injected into upper gut, heart, lung or trachea. In all cases, DIO-ChR2-EYFP was bilaterally injected into nodose ganglia. Gut (panel A), heart (B), trachea (C), and lung (D) terminal sites were restricted to distinctively separate sites within the solitary complex. Additional sections shown in Figures S2A–D. E. The upper gut of mice was injected with the retrograde construct CAV2-Cre-GFP, and nodose ganglia were bilaterally injected with a Cre-inducible AAV-Gs-coupled-mCherry chemogenetic designer receptor construct. CAV2-Cre-GFP and AAV-DIO-GsmCherry were detected in nodose ganglia bilaterally. Middle panel: mCherry expression is restricted to outermost nodose neurons (10×, Bar=100 M). Right panel: 40×, Bar=100 M. F. AAV-DIO-Gs-mCherry-infected terminals by injections into both right nodose (terminal fibers in medial NTS) and left nodose (terminal fibers in AP). G. CNO injections induce place preferences. During conditioning, in all sessions animals were administered I.G. with IntraLipid (0.3kcal, 0.6ml). CNO injections were then paired to the less preferred side of the cage. Left panel: Representative heat map showing the pre-test baseline (upper) and post-CNO preferences (lower). Right panel: Place preference for CNO-paired side, N=5; paired t-test t[4]=9.2, *p=0.001. H. Post-conditioning flavor preferences for flavors paired with 5% I.G. IntraLipid (0.3kcal, 0.6ml) + CNO vs. 5% I.G. IntraLipid + saline, N=5; paired t-test t[4]=9.5, *p=0.001. For one-sample t-tests against 50% (indifference) preferences: Pre-conditioning: t[4]=1.6, p=0.18; Post-conditioning, t[4]=7.5, Bonferroni-corrected p=0.004. I. CNO injections significantly enhanced dopamine release in dorsal striatum induced by 5% I.G. IntraLipid (0.3kcal, 0.6ml), N=6; Two-way RM-ANOVA CNO × sampling time interaction effect F[18,72]=7.59, *p<0.0001. AP= area postrema; DMV=dorsal motor nucleus of vagus; NTS=nucleus of the tractus solitarius. Data reported as mean±SEM.