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. 2018 Oct 19;8:15509. doi: 10.1038/s41598-018-33647-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NupC and NupG are the main transport systems involved in the incorporation of extracellular ADPG into envelope stressed E. coli cells. (A) Glycogen iodine staining of ∆rseA, ∆rseA∆nupC, ∆rseA∆nupG and ∆rseA∆nupC∆nupG cells, along with ∆rseA∆nupC cells ectopically expressing nupC and ∆rseA∆nupG cells ectopically expressing nupG (∆rseA∆nupC nupC e.e. and ∆rseA∆nupG nupG e.e., respectively) cultured in solid KM-ADPG. (B) ADPG consumption by BW25113 WT, ∆rseA, ∆rseA∆nupC, ∆rseA∆nupC bearing the empty pSU18 vector, ∆rseA∆nupG, ∆rseA∆nupG bearing the empty pSU18 vector, ∆rseA∆nupC∆nupG, ∆rseA∆nupC nupC e.e., ∆rseA∆nupG nupG e.e., cells cultured in liquid M9-glycerol-ADPG medium. Growth curves are shown in Supplementary Fig. S3G. (C) Glycogen iodine staining of WT and ∆nupC∆nupG cells cultured in solid KM-ADPG with or without 0.1% (w/v) DOC supplementation. (D) ADPG consumption by BW25113 WT and ∆nupC∆nupG cells cultured in liquid M9-glycerol-ADPG medium with or without 0.1% (w/v) DOC supplementation. Growth curves are shown in Supplementary Fig. S3H. In (B,D) values represent means ± SE obtained from four independent experiments with 3 replicates for each experiment.