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. 2018 Oct 19;8:15478. doi: 10.1038/s41598-018-33857-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of growth of wild-type and qcrABC strains on fumarate and nitrate under oxygen-limited conditions. (A) growth of wild-type (red lines and symbols) and qcrABC mutant (blue lines and symbols) in the absence (open symbols) or presence (closed symbols) of 20 mM sodium fumarate. The mutant does not have a growth defect on fumarate, consistent with the two fumarate reductases present in C. jejuni deriving electrons directly from the (methyl)menaquinol pool. (B) growth of wild-type (red line and closed symbols) and qcrABC mutant (green line and closed symbols) in the presence of 20 mM sodium nitrate. (C) nitrite concentrations were measured in samples taken from the same growth curve as in (B) as described in Experimental Procedures. The data shown are single representative growth curves from several that have been performed with independent inocula. In each case similar results were obtained.