Table 1.

Primers used in this study.

Name	Sequence 5′− 3′
qcrABC_ISA_F1F	GAGCTCGGTACCCGGGGATCCTCTAGAGTCTGGAGTTTTGCTTTTTAGTTTTG
qcrABC_ISA_F1R	AAGCTGTCAAACATGAGAACCAAGGAGAATGCTTCGTCTACTCTCAGATGTAGC
qcrABC_ISA_F2F	GAATTGTTTTAGTACCTAGCCAAGGTGTGCGCGTTCTGTTTGGTCTAAATTAC
qcrABC_ISA_F2R	AGAATACTCAAGCTTGCATGCCTGCAGGTCATAAAAATCATTACCTATATCATAATGACTTTT
Kan_F	ATTCTCCTTGGTTCTCATGTTTGACAGCTTAT
Kan_R	GCACACCTTGGCTAGGTACTAAAACAATTCAT
qcr_Screen_F	TTAAAATAAGTTTTTTTGCTTTGCT
qcr_Screen_R	AGTTTTTTAAGGGTATGTTCTATTTTGT
RT1186F	GAGAGTAGACGAAGCTTTATG
RT1186R	GTTCTAAGCTCTCCATCTTG
RT1185F	GTAGATTGGCTTGATCAAA
RT1185R	CAAGTGCAGTATCTGGTTT
RT1184F	GCTGTTGAAGATACTACTTTTG
RT1184R	CGATCTTTGCAACATCTAC
RT1183F	GAAACAAACCTGCTAAATTT
RT1183R	CCTTAGCCATTTCATCATA
RTgyrAF	ATGCTCTTTGCAGTAACCAAAAAA
RTgyAR	GGCCGATTTCACGCACTTTA

For RT-PCR primers the designation refers to the relevant gene (e.g. RT1186F is the forward primer for cj1186c).