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. 2018 Oct 19;9(11):1067. doi: 10.1038/s41419-018-1012-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Photomicrographs illustrate EZH2 with immunofluorescent staining of the kidney tissues collected at 48 h after IR injuring with or without 3-DZNep administration in C57/black mice. Scale bar, 20 µm. b Tubular cells with positive EZH2 staining were counted in 10 high-power fields and expressed as means ± SD. c, e Kidneys injured by IR or FA, respectively, tissue lysates were subjected to immunoblot analysis with specific antibodies against EZH2, H3K27me3, and ɑ-tubulin. d, f Expression levels of EZH2 and H3K27me3 were quantified by densitometry and normalized with ɑ-tubulin. Representative immunoblots are three samples in each group. Means with different superscript letters are significantly different from one another (P < 0.05)