Fig. 3. EZH2 inhibition decreases expression of neutrophil gelatinase-associated lipocalin (NGAL) in the kidney of IR-induced or FA-induced AKI.

a Photomicrographs illustrate immunofluorescent staining of NGAL in kidney tissues collected at 48 h after sham and IR injury with or without 3-DZNep administration in C57/black mice. b Tubules with positive NGAL staining were counted in 10 high-power fields and expressed as means ± SD. Scale bar, 20 µm. c, e Kidneys were injured by IR or FA, respectively, and tissue lysates were subjected to immunoblot analysis with specific antibodies against NGAL and ɑ-tubulin. d, f Expression levels of NGAL were quantified by densitometry and normalized with ɑ-tubulin. Data are means ± SD. Representative immunoblots are three samples in each group