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. 2018 Oct 19;8:15551. doi: 10.1038/s41598-018-33620-7

Figure 5.

Figure 5

TRPV1-expressing C-fibers are required for DAMGO-induced itch (A) Representative images and (B) quantification of intraepidermal Tuj1+ nerve fiber density per 100 μm of skin tissue, between PBS and RTX (three consecutive injections, one day apart: 30, 70 and 100 µg/mL) treated mice. Dotted line delineates the epidermis. Statistical analysis were performed using Mann Whitney U test (***p < 0.001 PBS versus RTX). (C) Scratching bouts evoked by intradermal injections of either PBS or DAMGO (200 µM) in WT (PBS; n = 4, DAMGO; n = 5) and RTX-treated mice (n = 6). Results are represented as Whiskers-dot plot (Min-Mean-Max) and each symbol represents an individual mouse. Statistical analysis were performed using Kruskall-Wallis followed by post-hoc Dunn’s test (*p < 0.05 PBS + DAMGO versus RTX + DAMGO).