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. 2018 Oct 19;8:15470. doi: 10.1038/s41598-018-33924-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Average amount of GAD67 protein and GABA production. (a) Densitometric analysis of Western Blot. Histograms report average amount of GAD67 normalized to alpha-Tubulin protein. Each histogram bar represents the mean value of triplicates. Cropped images of WB bands are reported below the graph, the original image of WB stain is given in Supplementary Fig. 5. (b) Histograms depict average amount of GABA normalized to total proteins amount measured. Each histogram bar represents the mean value of triplicates. Significance estimated by one-way ANOVA with LSD post hoc test (**P < 0.01).