Fig. 6.

DHX9 promotes the formation of pathological and non-pathological R-loops. a Diagram of the Β-actin locus depicting relative position of primer pairs used for qPCR in DNA–RNA immunoprecipitation (DRIP) and chromatin immunoprecipitation (ChIp) experiments. Exons are depicted as red boxes. b DRIP analysis at the Β-actin locus using S9.6 antibody. Quantitative PCR was performed using primers to the indicated regions of the Β-actin locus. Data are represented as fold enrichment compared to control immunoprecipitations. Data are an average of three independent experiments. c DRIP analysis of RNA–DNA hybrid at centromeric and flanking arm regions of chromosome 1 in cells knocked down for DHX9. Samples were treated with RNaseH1 to remove DNA–RNA hybrid are indicated