Fig. 8.

DHX9 associates with different phosphorylated forms of RNA Polymerase II. a Western blot of RNA Pol II and DHX9 immunoprecipitated from HeLa cells with antibodies specific for phosphor-serine 2 (S2P) and phosphor-serine 5 (S5P) forms of RNA Pol II. Where indicated cells were transfected with siRNA against a scrambled DNA sequence (siControl) or against SFPQ (siSFPQ8, siSFPQ9). b Expression of siRNA-resistant myc-SFPQ in SFPQ knockdown cells promotes the association of DHX9 with RNA Pol II S5P and diminishes its association with S2P. c Chromatin immunoprecipitation of DHX9 at the Β-actin locus. Data are depicted as fold enrichment over the control IP. qPCR of IP samples was performed for the primer pairs described in Fig. 6a. Means and s.e.m. are plotted and data are an average from three independent replicates. d Chromatin immunoprecipitation of RNA Pol II S2P at the Β-actin locus as described in c