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. 2018 Oct 19;8:15472. doi: 10.1038/s41598-018-33932-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of SB77 on HTM cell viability by MTT assay. (A) HTM cells treated with SB77 showed more than 85% (Mean percentage viability: 87.8 ± 5.9) cell viability at the studied concentrations (0.1–100 µM). The values are represented as mean ± SD. Experiments were conducted in triplicate. (B) Terminal deoxyribonucleotidyl transferase (TdT) - mediated fluroscein-16-dUTP nick end-labelling (TUNEL) staining on HTM cells after treatment with DNAse I (i), vehicle control (ii), SB77 (iii) and Y27632 (iv) for 24 h. TUNEL positive cells (green) were detected only in HTM cells treated with DNase I (i) (Positive control). No TUNEL positive cells were detected in other treatment groups. Cell nuclei were stained with DAPI (blue). A detailed protocol for MTT and TUNEL assay was given in method section.