Table 2.

Effects of substance-P (10 µM) on biophysical properties of the L-type calcium current (I_Ca,L) and the transient outward current (I_to)

	ICa,L-Control	ICa,L-Sub-P	n	Ito-Control	Ito-Sub-P	n
Current density (pA/pF)	−21.3 ± 3.3	−16.6 ± 2.3a	6	9.7 ± 2.9	8.9 ± 2.8	5
Act i vat i on
V1/2, mV	−15.4 ± 0.8	−17.2 ± 1.4	6	−7.0 ± 7.4	−15.1 ± 3.3	5
k, mV	6.8 ± 0.5	7.0 ± 0.7	6	14.2 ± 0.9	9.8 ± 0.1a	5
I nact i vat i on
V1/2, mV	−28.9 ± 1.3	−31.2 ± 2.3	6	−32.5 ± 3.4	−35.9 ± 2.8a	5
k, mV	4.4 ± 0.3	4.9 ± 0.3	6	−5.9 ± 0.9	−5.4 ± 0.2	5
I nact i vat i on t i me course
τfast, ms	4.4 ± 0.6	4.9 ± 0.7	6	39.6 ± 13.2	41.3 ± 8.1	5
τslow, ms	28.0 ± 3.5	28.2 ± 3.4	6	131.1 ± 33.7	114.6 ± 16.2	5

All values are mean ± SEM. Two-way repeated measures ANOVA followed by pairwise comparison using the Student–Newman–Keuls method = p < 0.05; Compared to control = ^a

Sub-P: substance-P, V_1/2: voltage of half-maximal (in)activation, k: slope factor, t_fast and t_slow: fast and slow time constants of inactivation