Fig. 1. miR-495-3p is downregulated in GC and inhibits MDR and proliferation of GC MDR cells.

a Real-time PCR detected the relative expression level of miR-495-3p in peri-tumor tissue and gastric cancer tissue in 15 paired samples. b The expression level of miR-495-3p in normal gastric epithelial cell line, five gastric cancer cell lines and GC MDR cells. c Four chemotherapeutic drugs (c1 adriamycin; c2 cisplatin; c3 fluorouracil; c4 vincristine) with indicated concentration gradient were added to the medium of SGC7901/ADR transfected with miR-495-3p mimics or nc. Then, cell survival was measured using the MTT assay. d Colony formation assays were performed to evaluate the proliferative functions of GC MDR cells transfected with miR-495-3p mimic or nc. 10³ cells per well were seeded initially in 6-well plates. e Representative images of tumors 4 weeks after subcutaneous injection of 10⁷ SGC7901/ADR transfected with lenti-NC or lenti-miR-495-3p into the right flank of the nude mice. (Scale bar = 1 cm). All experiments were performed in triplicates. All values were expressed as mean ± SD, n = 3 for each group. *P < 0.05, **P < 0.01, ***P < 0.001