Fig. 4.

GRP78 expression is increased and associated with poor outcomes in GC. a Real-time PCR shows the expression of GRP78 in 15 paired gastric cancer samples as compared to adjacent tumor tissues. b Western blotting indicates the protein level of GRP78 in 13 paired gastric cancer tissues and corresponding adjacent tumor tissues (C is carcinoma, N is normal tissues). Data from TCGA show the transcript per million of GRP78 in normal and primary GC tissues (c) based on individual cancer stages (d) and tumor grades (e). f The expression of GRP78 in normal gastric epithelial cell line and five gastric cancer cell lines and GC MDR cells by Real-time PCR. All values expressed as mean ± SD, n = 3 for each group. *P < 0.05, **P < 0.01, ***P < 0.001