Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 19;8:15467. doi: 10.1038/s41598-018-33769-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

SHIP1-deficiency compensates for signalling defects in BTK-deficient BMMCs. (a) WT, Btk−/−, Ship1−/−, and DKO BMMCs or (b) WT and Ship1−/− BMMCs, pre-treated with 0.3 µM Ibrutinib or vehicle (DMSO) for 30 min, were left untreated (con) or stimulated with suboptimal and optimal concentrations of Ag (DNP-HSA [DNP]) for 1 and 5 minutes. Whole-cell lysates were subjected to Western Blot analysis with the indicated antibodies against P-IKKα/β (Ser176/Ser177), P-PKB (Ser473), P-ERK1/2 (Thr202/Tyr204), P-p38 (Thr180/Tyr182), P-IκBα (Ser32), and p85 (loading control). Comparable results were obtained in at least three experiments with different BMMC cultures. Fine vertical lines were inserted to allow for better discrimination of cells/conditions. Because proteins of comparable size were analysed, the same lysates were separated on two gels with an anti-p85 loading control on each gel (a; marked with/without asterisk).