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. 2018 Oct 19;8:15552. doi: 10.1038/s41598-018-33710-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of pore size on SH-SY5Y migration and neurite extension through the membrane. (A) Images show the lower non-seeded side of the microporous membranes after 5 days in culture, following removal of cells and neurites from the upper side of the membranes. (B) Cell migration plot shows counts of in-focus DAPI stained cell nuclei on the lower side of the membrane. Neurite plot shows the mean neurites on the lower side of the membrane in comparison to the 0.8 µm pore diameter membranes and after normalisation against differences in membrane porosity. Plots show the means ± SD with dots representing fields of view (cell migration: n = 7, neurites: n = 12). p > 0.05 (*), p > 0.0001 (****), “not significant” (ns). Scale bar(50 µm).