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. 2018 Oct 19;8:15552. doi: 10.1038/s41598-018-33710-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Neurite growth of spatially separated SH-SY5Y neural populations across a 1.2 µM membrane. (A) Schematic demonstrating the membrane separating EGFP expressing (lower layer) and mCherry expressing (upper layer) neurons. (B–D) Images of the lower membrane. Arrows highlight: (p) the upper layer neurites emerging through pores, (g) upper layer neurites co-localised with lower layer neurites, and (i) upper layer neurites co-localised on lower layer neural soma. Scale bars (B: 50 µm) and (C, D: 10 µm).