Figure 3.

Increased expression of NKG2D ligands on dendritic cells (DCs) in NKG2D-deficient mice, which is enhanced during respiratory syncytial virus (RSV) infection. A and B, Rae1 and Mult-1 expression on mediastinal lymph node (mLN) DCs were measured by flow cytometry 2 days after RSV infection. Single lung cell suspension was prepared from mice at 2 days after RSV infection and stained with Rae1 and Mult-1 (C and D). Arrows point to cells that are positive for CD11c and Rae1 or Mult-1 staining (Blue: 4′,6-diamidino-2-phenylindole; Green: CD11c; Red: Rae 1/Mult-1). Data are representative of 4 independent experiments with n = 6–8 mice. *P < .05 compared to KO control. #P < .05 compared to WT RSV. Abbreviations: KO, knockout; RSV, respiratory syncytial virus; WT, wild-type.