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CRISPRO pipeline. a Dense mutagenesis of protein coding sequence by pooled CRISPR screening approach. Single guide RNAs target every possible PAM within the coding sequence of a set of genes. Guide RNAs are mapped to the two amino acids closest to the nuclease (e.g., Cas9) cleavage site. b Overview of the CRISPRO pipeline. Two input options are either FASTQ files or a precalculated score file (blue). Example data shown for MAP2K1 [8], PDB ID 4MNE
