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. 2018 Oct 20;11:285. doi: 10.1186/s13068-018-1272-z

Fig. 4.

Fig. 4

Sesquiterpene production with the MVA pathway and promoter engineering. a Plasmid architecture. A two-plasmid system for sesquiterpene production. Plasmid 1 contains MVA pathway genes producing FPP from acetyl-CoA either under a PlacUV5 (inducible) or an FPP-responsive promoter PgadE (dynamic) with a medium-copy origin (p15A) and chloramphenicol resistance gene. Plasmid 2 contains a sesquiterpene synthase gene under either Ptrc, PT7 or an FPP-responsive promoter PrstA with a high-copy origin (colE1) and ampicillin resistance gene. b Epi-isozizaene production. c Pentalenene production. d α-isocomene production. Titers of compounds were measured at 24, 48 and 72 h. Promoters for both plasmid 1 and plasmid 2 are listed to describe strains as well as strain names in x-axis. Data represent averages of three biological replicates with standard deviation as error bars