Table 1.
Description of E. coli base strains, plasmids and gene clusters used in this study
| Plasmid reference | Plasmid name | Relevant genotype | References | |
|---|---|---|---|---|
| JBEI-2704 | placUV5-MevT-MBIS | p15A, CmR | [44] | |
| JBEI-2872 | pgadE-MevT-MBIS | p15A, CmR | [25] | |
| JBEI-15857 | pTrc99a-EIZS | ColE1(pBR322) ori, AmpR | This study | |
| JBEI-15862 | pTrc99a-coEIZS | ColE1(pBR322) ori, AmpR | This study | |
| JBEI-15867 | pTrc99a-coPentS | ColE1(pBR322) ori, AmpR | This study | |
| JBEI-15865 | pTrc99a-coMrTPS2 | ColE1(pBR322) ori, AmpR | This study | |
| JBEI-15856 | pTrc99a-his-EIZS | ColE1(pBR322) ori, AmpR, his tag | This study | |
| JBEI-15855 | pTrc99a-his-coEIZS | ColE1(pBR322) ori, AmpR, his tag | This study | |
| JBEI-15854 | pTrc99a-his-coPentS | ColE1(pBR322) ori, AmpR, his tag | This study | |
| JBEI-15853 | pTrc99a-his-coMrTPS2 | ColE1(pBR322) ori, AmpR, his tag | This study | |
| JBEI-15849 | prstA-coEIZS | ColE1(pBR322) ori, AmpR | This study | |
| JBEI-15863 | prstA-coPentS | ColE1(pBR322) ori, AmpR | This study | |
| JBEI-15864 | prstA-coMrTPS2 | ColE1(pBR322) ori, AmpR | This study | |
| JBEI-15866 | pBbE7a-coEIZS | ColE1(pBR322) ori, AmpR | This study | |
| JBEI-15858 | pBbE7a-coPentS | ColE1(pBR322) ori, AmpR | This study | |
| JBEI-15859 | pBbE7a-coMrTPS2 | ColE1(pBR322) ori, AmpR | This study | |
| JBEI-15861 | pRSLeu2d-coPentS | 2μ ori, pBR322 ori, Leu2d | This study |
| Strain name | Description | References | |
|---|---|---|---|
| CL1601 | JBEI-2704 + JBEI-15862 | This study | |
| CL1602 | JBEI-2704 + JBEI-15849 | This study | |
| CL1603 | JBEI-2872 + JBEI-15849 | This study | |
| CL1604 | JBEI-2872 + JBEI-15862 | This study | |
| CL1605 | JBEI-2704 + JBEI-15866 | This study | |
| CL1606 | JBEI-2872 + JBEI-15866 | This Study | |
| CL1607 | JBEI-2704 + JBEI-15867 | This study | |
| CL1608 | JBEI-2704 + JBEI-15863 | This Study | |
| CL1609 | JBEI-2872 + JBEI-15863 | This study | |
| CL1610 | JBEI-2872 + JBEI-15867 | This study | |
| CL1611 | JBEI-2704 + JBEI-15858 | This study | |
| CL1612 | JBEI-2872 + JBEI-15858 | This study | |
| CL1613 | JBEI-2704 + JBEI-15865 | This study | |
| CL1614 | JBEI-2704 + JBEI-15864 | This study | |
| CL1615 | JBEI-2872 + JBEI-15864 | This study | |
| CL1616 | JBEI-2872 + JBEI-15865 | This study | |
| CL1617 | JBEI-2704 + JBEI-15859 | This study | |
| CL1618 | JBEI-2872 + JBEI-15859 | This study | |
| CL1619 | S. cerevisiae EPY300 harboring JBEI-15861 | This study |