Fig. 1.
PA sensitivity and glucan synthase activity of C. albicans. Sensitivity of C. albicans SC5314 (wild type), NR3 (containing a point mutation in FKS1) and S. cerevisiae S288C to PA [A] and caspofungin [C]. Samples of 2.5 μl from the wells of the MIC plates were spotted on YPD plates and incubated at 30 °C for 24 h [B & D]. Error bars are SEM (n = 3). [E] Membranes of SC5314, NR3, and S288C were prepared from logarithmic-phase cells and glucan synthase (GS) activities were measured after solubilization of purified membrane with 0.4% Brij-35. Final concentrations of PA were 8, 16, 32, 64, 128, 256, and 512 μg/mL. All measurements were performed in triplicate. Plots of S288C, SC5314, and NR3 separately show mean values ± SD (n = 3). The half maximal inhibitory concentration (IC50GS) of S288C, SC5314 and NR3 were 206, 194 and 220 µg/ml, respectively.