Fig. 7.

Cell wall glucan contents in different Candida species and S. cerevisiae. [A and B] MIC of PA was calculated with exogenous β-1,3-glucan (βGlu) at 0.25 mg/ml or 0.5 mg/ml. C. albicans wild type [i] and S. cerevisiae [ii] were treated with PA only or PA with βGlu. Results are expressed as percentage of growth relative to the control (DMSO). Error bars = SEM (n > 3). * p < 0.05. 2way ANOVA test. [B] Correlation between glucan content and PA IC₅₀ values of different Candida species. Cells were grown exponentially for 6 h in YPD and washed with PBS. Cells were then fixed with 4% formaldehyde. Aniline Blue (25 µg/ml) was used to stain cell wall β-glucan. Fluorescence levels of individual cells (1 × 10⁴) were measured by FACS. The IC values were calculated using LOWESS analysis based on the sensitivity assay.