The M. trichosporium OB3b MMO and Mbn operons. (A) The pMMO operon. The order of genes in proteobacterial particulate methane monooxygenase and ammonia monooxygenase is p/amoCAB; other gene orders occur in some related monooxygenase systems. (B) The sMMO operon. The core sMMO operon (mmoXYBZDC) is found adjacent to mmoR and mmoG, but the order of these genes may vary, and additional operon members are present in some species. (C) The Mbn operon, including a core comprising biosynthesis and export genes (mbnABCMN), a group of import genes (mbn(E)IRT), and two genes of unknown function (mbnPH). (D) Overlap between sMMO, pMMO/AMO, and Mbn operons in organisms present in the JGI/IMG database. All three operons occur independently; no one operon appears to be necessary for either of the others to function.