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. 2018 Sep 29;33(11):2107–2121. doi: 10.1093/humrep/dey289

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© The Author(s) 2018. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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DMRT1 knockdown in second-trimester human fetal testis alters expression of genes involved in testicular and ovarian development. RT-qPCR analysis of genes associated with development of (A) Sertoli (B) granulosa and (C) germ cells in second-trimester human fetal testes tissue culture for 13 days after an initial 24 h exposure to scrambled miRNA, miR536 or miR641. Expression was normalized to RPS29 (n = 2–3 per treatment group, *P < 0.05, **P < 0.01, ***P < 0.001). Graphs show mean ± SEM. Data analyzed using a linear mixed effects model in R.