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. 2018 Oct 4;33(11):2060–2073. doi: 10.1093/humrep/dey290

Figure 5.

Figure 5

Effects of the ovarian steroid hormones (17β-estradiol and progesterone, E2P4) on AQP3 in expression RL95-2 cells. (A) Real-time PCR analysis of AQP3 mRNA in RL95-2 cells (a). Western blot detection of AQP3 protein in RL95-2 cells (b, c). GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Each experiment was repeated seven times. (B) Knockdown of AQP3 reduced E2P4-induced cell migration (B, a(0 h), b(48 h), c) and invasion (Bd, e). All images are magnified ×100. Bar = 100 μm. The data are presented as the means ± SD, *P < 0.05 and **P < 0.01 (Student’s t-test). Experiments were repeated six times. Estradiol concentration: 10 nM; progesterone concentration: 1 μM; E2P4 concentration: 10 nM estradiol and 1 μM progesterone.